Optimization changed over time - optimization today does not address CPU cycle/instruction, rather reduced memory transaction are the key to success. Keep the code small and keep the code in the cacheline and reduce memory loads. A cache miss is equivalent to 100 instructions during a CPU stall. Therefore keeping the @.text@ footprint small can boost your application more then some sophisticated CPU tweaks. Pahole provides a feeling how the @.data@ segment is constructed, how structs are aligned in memory, it provides information if holes in structs exists and how the linker align the data at boundaries. This scratches the surface of optimization techniques and the kernel uses highly sophisticated techniques to optimize for memory transactions: false sharing of elements which are used mainly readonly (put it in another @.data@ section), align data on different cachelines to avoid false sharing, UNinline functions to reduce the memory footprint and so on.
One of the best books to understand this kind of optimization is called "UNIX Systems for Modern Architectures - Symmetric Multiprocessing and Caching for Kernel Programmers" from Curt Schimmel. This is by far the best book in this area. Beside this the optimization manuals from AMD and INTEL are worth to read it too, because they provide a in deep understanding about the actual processor specific tweaks.